Contribution to GENOME NEWS (UK HUMAN GENE MAPPING PROJECT NEWSLETTER) sept 1991 p. 32
Department of Molecular and Cellular Physiology, AFRC Institute of Animal Physiology and Genetics research, Cambridge Research Station, Babraham Hall, Cambridge CB2 4AT
Dr. E.M. Tucker Dr. J.R Miller Dr. A. Bouvet Dr. B.A. Konfortov Mrs C. Langford
Tel:(0223) 832312 ext:243, 255 FAX: (0223) 836122
We participate in the European Pig Genome Mapping Project (pigMap), whose aim is to generate both a physical and a genetic map of the pig genome. Our work involves defining the pig flow karyotype using a Dual-laser FACS machine. We intend to utilise the sorted chromosomes for physical gene mapping via dot blot hybridization and PCR amplification. A panel of pig-mouse hybrid cell lines has been constructed and will be used for synteny mapping.
A chromosome-specific library for pig chromosome 1 has been constructed specificity of the library. A tandem repeat family which hybridises to the centromeres of all pig chromosomes except the Y has been isolated and characterized. This sequen.ce family has a chromosome specific distribution of restriction enzyme sites. Both the above projects have been carried out with Preben Thomsen (Copenhagen Veterinary College). LINE priming (Line PCR) is being assessed as a possible source of polymorphic markers. The distribution of SINE and LINE sŽquences in the pig genome is being studied in collaboration with Preben Thomsen and William Davies (Norwegian Veterinary College, Oslo).
A bovine comparative gene mapping project involving the bovine genes corresponding to those on the sort arm of human chromosome 1 7 has just been initiated.